Journal: The FASEB Journal
Article Title: Isocitrate dehydrogenase 1 upregulation in urinary extracellular vesicles from proximal tubules of type 2 diabetic rats
doi: 10.1096/fj.202400371r
Figure Lengend Snippet: FIGURE 5 Lectin affinity enrichment of uEVs from LETO and OLETF rats. (A) Schematic representation of lectin affinity enrichment. The uEVs isolated by ultracentrifugation were mixed with biotinylated lectin or PBS (as control) and then mixed with streptavidin magnetic beads. The uEVs attached to the beads were eluted by eluting sugar. (B) Immunoblots of IDH1, proximal tubule markers (NHE3, SGLT2, and AQP1), collecting duct marker (AQP2), and EV markers (Alix, TSG101, and CD9) in uEVs enriched by Lens culinaris lectin (LCA) from LETO and OLETF rats. (C) Staining of the renal cortex, outer medulla, and inner medulla with IDH1, LCA, and DAPI. The scale bar is 100 μm. Red, LCA. Green, IDH1. Blue, DAPI.
Article Snippet: Mouse anti- Alix monoclonal antibody, mouse anti- AQP1 monoclonal antibody, mouse anti- AQP2 monoclonal antibody, mouse anti- SGLT2 monoclonal antibody, mouse anti- NHE3 monoclonal antibody, mouse anti- CD81 monoclonal antibody, mouse anti- TSG101 monoclonal antibody, and mouse anti- THP monoclonal antibody were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Techniques: Isolation, Control, Magnetic Beads, Western Blot, Marker, Staining